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Ryosuke Kojima, Dr. Hideo Takakura, Prof. Takeaki Ozawa, Yukio Tada, Prof. Tetsuo Nagano and Prof. Yasuteru Urano Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo Angewandte Chemie International Edition 52

Version of Record online: 4 DEC 2012 | DOI: 10.1002/anie.201205151

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Shine on: A new rational design strategy for near-infrared-emitting firefly luciferins that are available in vivo has been developed using intramolecular bioluminescence resonance energy transfer (BRET). The emission wavelength could be freely tuned by the choice of BRET acceptor, and NIR bioluminescence could be detected in living cells and mice without the need for luciferase manipulation.

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