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Dr. Lin Li, Xiaoqin Shen, Prof. Dr. Qing-Hua Xu and Prof. Dr. Shao Q. Yao A Switchable Two-Photon Membrane Tracer Capable of Imaging Membrane-Associated Protein Tyrosine Phosphatase Activities Angewandte Chemie International Edition 52

Version of Record online: 4 OCT 2012 | DOI: 10.1002/anie.201205940

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When I look into your cells: A two-photon dye (Flu7) was developed (see scheme), which strongly fluoresces only upon selective binding to the plasma membrane of mammalian cells. Upon addition of a UV- and phosphatase-controlled quencher (Q12), the system exhibits ON/OFF/ON fluorescence switching and can be used to image membrane-associated receptor-like protein tyrosine phosphatase (RPTP) activity.

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