Deepak K. Prusty, Minseok Kwak, Jur Wildeman and Prof. Dr. Andreas Herrmann Modular Assembly of a Pd Catalyst within a DNA Scaffold for the Amplified Colorimetric and Fluorimetric Detection of Nucleic Acids Angewandte Chemie International Edition 51
Phosphine ligands were covalently tethered to short oligonucleotide strands such that active palladium complexes formed in the presence of a specific nucleic acid target. This catalytic center on the double-stranded DNA then converted water-soluble iodo-BODIPY dyes, present in excess, into highly emissive deiodinated reporters (see picture). This approach is suitable for the rapid colorimetric or fluorimetric detection of nucleic acid targets with a lower detection limit of just 10 pM.
Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf