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Frank Wintermann and Siegfried Engelbrecht Reconstitution of the Catalytic Core of F-ATPase (αβ)3γ from Escherichia coli Using Chemically Synthesized Subunit γ Angewandte Chemie International Edition 52

Version of Record online: 4 DEC 2012 | DOI: 10.1002/anie.201206744

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Hooking up: Subunit γ of an F-ATPase with 11 point mutations was ligated chemically from six peptides and then reconstituted with further subunits of the (αβ)3γ catalytic core complex. The biologically active protein with 286 residues currently represents the longest nonredundant polypeptide chain synthesized by chemical means. The yields of the five consecutive ligations, especially the last three, dropped the total yield to only 0.005 %.

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