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Erkin Kuru, H. Velocity Hughes, Dr. Pamela J. Brown, Edward Hall, Srinivas Tekkam, Prof. Felipe Cava, Prof. Miguel A. de Pedro, Prof. Yves V. Brun and Prof. Michael S. VanNieuwenhze In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with Fluorescent D-Amino Acids Angewandte Chemie International Edition 51

Article first published online: 10 OCT 2012 | DOI: 10.1002/anie.201206749

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Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC=7-hydroxycoumarin, NBD=7-nitrobenzofurazan, TAMRA=carboxytetramethylrhodamine.

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