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Anushikha Thakur, Dr. Kousik Chandra, Abhinav Dubey, Dr. Patrick D'Silva and Prof. Hanudatta S. Atreya Rapid Characterization of Hydrogen Exchange in Proteins Angewandte Chemie International Edition 52

Version of Record online: 22 JAN 2013 | DOI: 10.1002/anie.201206828

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Kinetics and thermodynamics of amide hydrogen exchange in proteins can be investigated with two-dimensional 13CO–15N NMR correlation experiments (see picture). The spectra are acquired with high resolution and sensitivity. A single type of experiment on one sample serves to characterize hydrogen–deuterium fractionation factors and hydrogen-exchange rates that span three orders of magnitude.

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