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Satoshi Kubo, Dr. Noritaka Nishida, Yuko Udagawa, Osamu Takarada, Dr. Shinji Ogino and Prof. Dr. Ichio Shimada A Gel-Encapsulated Bioreactor System for NMR Studies of Protein–Protein Interactions in Living Mammalian Cells Angewandte Chemie International Edition 52

Version of Record online: 29 NOV 2012 | DOI: 10.1002/anie.201207243

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Staying alive: The major limitation of in-cell NMR spectroscopy methods is the occurrence of cell death during the NMR measurement. To overcome this problem, a bioreactor was utilized that can perfuse the cells in the NMR tube, thereby maintaining the conditions for more than 5 h. By using the bioreactor, the binding site of an externally introduced protein for an endogenous molecule in HeLa S3 cells was identified.

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