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Joseph J. Bellucci, Dr. Miriam Amiram, Dr. Jayanta Bhattacharyya, Prof. Dewey McCafferty and Prof. Ashutosh Chilkoti Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides Angewandte Chemie International Edition 52

Version of Record online: 19 FEB 2013 | DOI: 10.1002/anie.201208292

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Sorting it out: Applied in tandem, elastin-like polypeptides (ELPs, see scheme) and the Sortase A (SrtA) transpeptidase provide a method for chromatography-free purification of recombinant proteins and optional, site-specific conjugation of the protein to a small molecule (yellow). This system provides an efficient mechanism for generating bioactive proteins at high yields and purities.

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