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Prof. Qunzhao Wang, Prof. Melanie A. Priestman and Prof. David S. Lawrence Monitoring of Protein Arginine Deiminase Activity by Using Fluorescence Quenching: Multicolor Visualization of Citrullination Angewandte Chemie International Edition 52

Article first published online: 21 JAN 2013 | DOI: 10.1002/anie.201208464

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Colorful: The protein arginine deiminases are members of the enzyme family that catalyze posttranslational histone modification and consequent changes in gene expression. A visual readout of catalytic activity was developed that yields large fluorescence changes across the visible spectrum. The use of different fluorophores (see picture) enables the simultaneous multicolor monitoring of a mixture of histone-modifying enzymes.

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