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Prof. Rainer Cramer, Alexander Pirkl, Prof. Franz Hillenkamp and Prof. Klaus Dreisewerd Liquid AP-UV-MALDI Enables Stable Ion Yields of Multiply Charged Peptide and Protein Ions for Sensitive Analysis by Mass Spectrometry Angewandte Chemie International Edition 52

Version of Record online: 22 JAN 2013 | DOI: 10.1002/anie.201208628

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All charged up: High and prolonged yields of multiply charged peptide and protein ions can be formed in MALDI mass spectrometry using liquid UV-MALDI matrices and a heated ion-transfer tube. The key features are low laser energies of 1–10 μJ, resulting in fluences of less than 200 to 2000 J m−2 and low sample ablation, high sensitivity, and continuous ion generation over tens of thousands of laser shots.

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