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Tae-Kyung Yu, Young-Joo Yun, Ko On Lee and Prof. Jeong-Yong Suh Probing Target Search Pathways during Protein–Protein Association by Rational Mutations Based on Paramagnetic Relaxation Enhancement Angewandte Chemie International Edition 52

Article first published online: 12 FEB 2013 | DOI: 10.1002/anie.201208688

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Not just a random encounter: Protein–protein association involves short-lived encounter complexes that can be productive or non-productive according to their roles in the specific complex formation (see scheme). A study of mutant proteins forming a complex based on NMR paramagnetic relaxation enhancement demonstrates that productive encounter complexes can be directly monitored and located quantitatively.

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