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Dr. Jens Binschik and Prof. Dr. Henning D. Mootz Chemical Bypass of Intein-Catalyzed N–S Acyl Shift in Protein Splicing Angewandte Chemie International Edition 52

Article first published online: 6 MAR 2013 | DOI: 10.1002/anie.201208863

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Spliced: An N-methyl group was introduced at the scissile peptide bond in a semisynthetic intein precursor. With the chemical modification, thioester formation and protein splicing was observed in the absence of the otherwise critical block B histidine. This finding shows the role of the histidine in ground-state destabilization and rules out other previously proposed roles for this residue in the protein splicing pathway.

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