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Dr. Pramodh Vallurupalli and Prof. Lewis E. Kay Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy Angewandte Chemie International Edition 52

Version of Record online: 28 FEB 2013 | DOI: 10.1002/anie.201209118

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Seeing the invisible: A 13CO NMR chemical exchange saturation transfer (CEST) experiment for the study of “invisible” excited protein states with lifetimes on the order of 5–50 ms has been developed. The 13CO chemical shifts together with those obtained from fits of 15N CEST profiles establish that the A39G FF domain folds via a similar compact intermediate (I) as the wild-type protein (F and U=native and unfolded states).

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