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Dr. Krishnappa Jagadish, Dr. Radhika Borra, Vanessa Lacey, Subhabrata Majumder, Dr. Alexander Shekhtman, Dr. Lei Wang and Dr. Julio A. Camarero Expression of Fluorescent Cyclotides using Protein Trans-Splicing for Easy Monitoring of Cyclotide–Protein Interactions Angewandte Chemie International Edition 52

Article first published online: 15 JAN 2013 | DOI: 10.1002/anie.201209219

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Going in circles: Expression of cyclotides containing non-natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing p-azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper-free click chemistry to monitor the cyclotide–protein interactions.

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