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Yanbo Pan, Prof. Dr. Mingliang Ye, Dr. Liang Zhao, Kai Cheng, Mingming Dong, Chunxia Song, Hongqiang Qin, Dr. Fangjun Wang and Prof. Dr. Hanfa Zou N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics Angewandte Chemie International Edition 52

Article first published online: 5 JUL 2013 | DOI: 10.1002/anie.201303429

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Two times an enzyme: Trypsin was first used as a protease to catalyze the digestion of proteins and then as a ligase to catalyze the linking of isotopically labeled amino acids to the N termini of tryptic peptides for quantitative proteomics. Reliable and accurate quantification of proteins was demonstrated for model proteins as well as proteome samples.

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