E-mail a Wiley Online Library Link

Maren Dill, Moritz C. Baier, Prof. Dr. Stefan Mecking and Dr. Dominik Wöll Enhanced Accuracy of Single-Molecule Diffusion Measurements with a Photocleavable Energy-Transfer Dyad Angewandte Chemie International Edition 52

Version of Record online: 25 SEP 2013 | DOI: 10.1002/anie.201303658

Thumbnail image of graphical abstract

A photocleavable energy-transfer dyad was synthesized, characterized, and applied to single-molecule fluorescence microscopy. After photocleavage, a combination of independent two-color single-molecule tracking and analysis of single-molecule energy-transfer efficiencies allows the determination of the temporal evolution of the relative distances between both fragments from the nm to the μm scale. This gives access to a broad range of diffusion coefficients.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field