Maren Dill, Moritz C. Baier, Prof. Dr. Stefan Mecking and Dr. Dominik Wöll Enhanced Accuracy of Single-Molecule Diffusion Measurements with a Photocleavable Energy-Transfer Dyad Angewandte Chemie International Edition 52
A photocleavable energy-transfer dyad was synthesized, characterized, and applied to single-molecule fluorescence microscopy. After photocleavage, a combination of independent two-color single-molecule tracking and analysis of single-molecule energy-transfer efficiencies allows the determination of the temporal evolution of the relative distances between both fragments from the nm to the μm scale. This gives access to a broad range of diffusion coefficients.
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