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Jan Huisken Slicing embryos gently with laser light sheets BioEssays 34

Version of Record online: 6 MAR 2012 | DOI: 10.1002/bies.201100120

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Light sheet microscopy is ideally suited to in vivo imaging over long periods of time as it not only offers low photo-toxicity but also high image acquisition rates. One example of this technique is selective plane illumination microscopy (SPIM) which, for example, even allows imaging of entire embryos with isotropic resolution.

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