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Tom O. Delmont, Pascal Simonet and Timothy M. Vogel Mastering methodological pitfalls for surviving the metagenomic jungle BioEssays 35

Version of Record online: 11 JUN 2013 | DOI: 10.1002/bies.201200155

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Metagenomics is a powerful approach targeting environmental nucleic diversity but represents also a methodological jungle where pitfalls are challenging when quantitative observations are desired. Here we describe the effect of critical parameters (especially DNA extraction and annotation stringency) required to represent microbial communities function and structure in the metagenomic era.

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