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Jiajie Diao, Minglei Zhao, Yunxiang Zhang, Minjoung Kyoung and Axel T. Brunger Studying protein-reconstituted proteoliposome fusion with content indicators in vitro BioEssays 35

Article first published online: 29 APR 2013 | DOI: 10.1002/bies.201300010

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In vitro reconstitution assays are commonly used to study molecular mechanism of SNARE-mediated membrane fusion. The large difference between lipid and content mixing observed from single vesicle-vesicle assay simultaneously monitoring both lipid and content exchange, calls into question proteoliposome assays that only rely on lipid-mixing reporters to assess fast fusion.

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