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Dr. Juan A. Hernández Bort, Matthias Hackl, Helga Höflmayer, Vaibhav Jadhav, Eva Harreither, Niraj Kumar, Wolfgang Ernst, Johannes Grillari and Nicole Borth Dynamic mRNA and miRNA profiling of CHO-K1 suspension cell cultures Biotechnology Journal 7

Version of Record online: 5 AUG 2011 | DOI: 10.1002/biot.201100143

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The cellular transcriptome is dynamic, changing in response to external and internal stimuli and thus reflecting the current functional capacity of cells as well their ability to adapt to a changing environment. In this study, the authors use microarrays expression profiling to identify mRNA and miRNA that are differentially expressed during CHO-K1 suspension cell batch cultures. The results show the importance of time course experiments for comparison of phenotypes at different culture conditions.

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