Joseph A. Gredell, Christopher S. Frei and Dr. Patrick C. Cirino Protein and RNA engineering to customize microbial molecular reporting Biotechnology Journal 7
By following nature's example, ligand-responsive proteins and RNA molecules are now routinely engineered and incorporated into customized molecular reporting systems, such as biosensors. In this review, the authors compare recent efforts in the design and application of customized proteins, RNA molecules, and genetic circuits which control microbial phenotypes in response to specific small molecule stimuli. Examples include altering regulatory protein effector specificity, engineering new allosteric enzymes, and developing in vivo screening systems for novel riboswitches.
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