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Farhat Khan, Patricia M. Legler, Ryan M. Mease, Elizabeth H. Duncan, Elke S. Bergmann-Leitner and Dr. Evelina Angov Histidine affinity tags affect MSP142 structural stability and immunodominance in mice Biotechnology Journal 7

Version of Record online: 28 DEC 2011 | DOI: 10.1002/biot.201100331

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Histidine tags can influence protein expression levels, structural stability and immunodominance: Experimental evidence from the current study demonstrates that His tags have far reaching impact on protein helical content and stability, and influence immunodominance of humoral and cellular immune responses in mice. The overall advantages of improved expression level and ease of chromatography may be outweighed by the effect on protein structure and the diversion of host immune responses.

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