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Hsiao-Fen Grace Chang, Yun-Long Tsai, Chuan-Fu Tsai, Ching-Ko Lin, Pei-Yu Lee, Ping-Hua Teng, Chen Su and Dr. Chien-Chung Jeng A thermally baffled device for highly stabilized convective PCR Biotechnology Journal 7

Version of Record online: 7 FEB 2012 | DOI: 10.1002/biot.201100453

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Thermally baffled device can reduce the environmental influence on convective PCR amplification: Convective PCR is extremely sensitive to environmental temperature fluctuations, and is inherently unstable with limited applications. In this study the authors have designed a thermally baffled convective PCR device with thermal baffles, which significantly reduces the influence from environmental temperature fluctuations, while PCR amplification is carried out successfully in a short reaction time. With its simple design the thermally baffled PCR instrument can provide a powerful tool for point-of-care molecular diagnostics in the field.

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