Sibel Cetinel, H. Burak Caliskan, Deniz T. Yucesoy, A. Senem Donatan, Esra Yuca, Mustafa Urgen, Nevin G. Karaguler and Prof. Candan Tamerler Addressable self-immobilization of lactate dehydrogenase across multiple length scales Biotechnology Journal 8
Inorganic-binding peptide tags provide a singk-step approach for self-organization of enzymes on solid surfaces. In this article, gold-binding peptide (AuBP2) was genetically incorporated to L-lactate dehydrogenase (LDH). Enhanced enzymatic activity was observed on a variety of gold surfaces ranging from nanoparticles to electrodes. Redox catalysis efficiency obtained by the immobilized fusion enzyme suggests that the proposed peptide-mediated path could be extended to a variety of applications.
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