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Blake Erickson, Ming Fang, Prof. Joseph M. Wallace, Prof. Bradford G. Orr, Clifford M. Les and Dr. Mark M. Banaszak Holl Nanoscale structure of type I collagen fibrils: Quantitative measurement of D-spacing Biotechnology Journal 8

Version of Record online: 24 OCT 2012 | DOI: 10.1002/biot.201200174

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The distribution of Type I collagen fibril D-spacing provides important morphological information regarding Type I collagen in diseases such as early stages of osteoporosis and osteogenesis Imperfecta. In this article, the authors use Atomic Force Microscopy (AFM) imaging combined with two Dimensional Fast Fourier Transform (2D FFT) analysis to quantitatively assess Type I collagen fibril D-spacing. This methodology allows imaging and characterization of Type I collagen constituted biological tissues, hydrogels, and other collagen based biomaterials.

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