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Steven D. Branston, Cristina F.R.O. Matos, Robert B. Freedman, Colin Robinson and Eli Keshavarz-Moore Investigation of the impact of Tat export pathway enhancement on E. coli culture, protein production and early stage recovery Biotechnology and Bioengineering 109

Version of Record online: 12 DEC 2011 | DOI: 10.1002/bit.24384

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The E. coli Tat pathway has the potential to export commercially useful proteins. Uniquely, it exports folded proteins, but its native capacity is low. This article studies the fermentation and downstream processing performances of E. coli strains engineered to over-express the Tat pathway to remove this bottleneck. Fermentation experimentation showed increased growth and periplasmic protein in engineered strains. An ultra scale-down approach used to predict robustness and clarification behavior showed this was achieved with no compromise in downstream processing performance.

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