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Claudia Korneli, Christoph Josef Bolten, Thibault Godard, Ezequiel Franco-Lara and Christoph Wittmann Debottlenecking recombinant protein production in Bacillus megaterium under large-scale conditions—targeted precursor feeding designed from metabolomics Biotechnology and Bioengineering 109

Version of Record online: 31 JAN 2012 | DOI: 10.1002/bit.24434

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The presented strategy displays a valuable tool for targeted large scale process optimization. By correlating culture performance and intracellular metabolite availability in a reactor configuration mimicking large scale, bottlenecks for production of recombinant proteins caused by limitation of specific precursors can be easily identified. This is demonstrated by targeted feeding of five identified precursors which increased protein production under large scale conditions by 100%.

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