Daniel Pleissner and Niels T. Eriksen Effects of phosphorous, nitrogen, and carbon limitation on biomass composition in batch and continuous flow cultures of the heterotrophic dinoflagellate Crypthecodinium cohnii Biotechnology and Bioengineering 109
This paper describes biomass composition of phosphorous, nitrogen, and carbon limited continuous flow cultures of the heterotrophic dinoflagellate Crypthecodinium cohnii. Specific contents of phosphorous, protein, and starch were strongly affected by nutrient limitation, while the specific lipid content, observable by yellow fluorescence after Nile red staining, remained almost unaffected. Carbon was taken up and stored in lipids and starch whenever available. Production of lipids that are rich in docosahexaenoic acid, therefore does not depend on nutrient limitation in C. cohnii.
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