Vaibhav Jadhav, Matthias Hackl, Juan A. Hernandez Bort, Matthias Wieser, Eva Harreither, Renate Kunert, Nicole Borth and Johannes Grillari A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells Biotechnology and Bioengineering 109
This work presents the screening method for overexpression and functional analysis of miRNAs in CHO cells, which can be rapidly customized to test large numbers of miRNAs for cell engineering applications. The same procedure can be applied to different production cell lines for assaying specific responses. Thus the presented system represents a general platform to functionally screen candidates for rational cell factory design using miRNA, miR-sponges, siRNAs or mRNA overexpression along with detailed functional characterization.
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