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Germarie Sánchez-Pomales, Todd A. Morris, James B. Falabella, Michael J. Tarlov and Rebecca A. Zangmeister A lectin-based gold nanoparticle assay for probing glycosylation of glycoproteins Biotechnology and Bioengineering 109

Version of Record online: 17 APR 2012 | DOI: 10.1002/bit.24513

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The authors demonstrate a rapid glycoanalysis method by lectin binding to glycoprotein-modified gold nanoparticles detected using either simple instrumentation (e.g., DLS or spectrophotometry) or by visual inspection. The assay is applied to a model protein, RNase B and a therapeutic monoclonal antibody, rituximab. The versatility of this method is great due to the breadth of the assay design space and may prove useful for rapid, high-throughput detection of changes in glycan profiles of therapeutic glycoproteins in process development or process monitoring.

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