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Lars Kober, Christoph Zehe and Juergen Bode Development of a novel ER stress based selection system for the isolation of highly productive clones Biotechnology and Bioengineering 109

Version of Record online: 24 APR 2012 | DOI: 10.1002/bit.24527

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The novel selection system based on ER stress works as follows: Overexpression of secretory recombinant proteins (Step 1) causes GRP78 dissociation from various ER stress sensors (Step 2). Transcription factors (TF) are formed (simplified pathway shown in Step 3), the natural GRP78 promoter (Step 4) as well as an artificial d2eGFP Reporter construct (Step 5) is upregulated and high expressing clones can be identify and isolated by FACS.

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