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Nuttapol Tanadchangsaeng and Jian Yu Microbial synthesis of polyhydroxybutyrate from glycerol: Gluconeogenesis, molecular weight and material properties of biopolyester Biotechnology and Bioengineering 109

Article first published online: 8 JUN 2012 | DOI: 10.1002/bit.24546

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In poly(3-hydroxybutyrate) (PHB) biopolyester production from glycerol, lithotrophical utilization of glycerol (non-fermentative substrate) or gluconeogenesis is an essential part of the main metabolic pathway to provide sugars necessary to biosynthesis of cellular components. The authors found that under physiological stress, the sugar (glucose) released without utilization, resulting in a relatively lower cell growth rate and yield. The obtained PHB polymer possessed the lower molecular size corresponding to the cultivation time.

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