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Jae Seong Lee and Gyun Min Lee Rapamycin treatment inhibits CHO cell death in a serum-free suspension culture by autophagy induction Biotechnology and Bioengineering 109

Version of Record online: 7 JUN 2012 | DOI: 10.1002/bit.24567

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In this study, the role of autophagy in CHO host cells (DG44) and antibody producing rCHO cells during serum-free suspension culture are investigated using a representative autophagy inducer, rapamycin. The increased autophagy level by rapamycin treatment can delay the apoptosis induction and viability drop, resulting in improved production of antibody-producing rCHO cells. These results imply a positive role of autophagy and the usefulness of pro-autophagy engineering in CHO cell culture.

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