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John P. Welsh, Yuan Lu, Xiao-Song He, Harry B. Greenberg and James R. Swartz Cell-free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens Biotechnology and Bioengineering 109

Version of Record online: 16 AUG 2012 | DOI: 10.1002/bit.24581

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In order to more effectively combat pandemic influenza threats, we need rapid and robust vaccine production methods. Here Welsh and coworkers describe rapid, cost-effective methods for producing the immunoprotective head domain of the influenza surface protein hemagglutinin (HA) using E. coli-based cell-free protein synthesis. After introducing targeted mutations, this protein domain was produced at high yields in either monomeric or trimeric form. The trimer binds sialic acid with the expected affinity suggesting proper folding and full functionality.

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