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Isabel Pardo, Ana Isabel Vicente, Diana M. Mate, Miguel Alcalde and Susana Camarero Development of chimeric laccases by directed evolution Biotechnology and Bioengineering 109

Version of Record online: 11 JUL 2012 | DOI: 10.1002/bit.24588

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Chimeric laccases have been obtained by in vitro and in vivo DNA shuffling of two fungal high redox potential laccases (from Pycnoporus cinnabarinus and PM1 basidiomycetes). The laccase hybrids disclosed in one single round of evolution (using a dual high-throughout screening assay) are active on phenolic and non-phenolic polar substrates and possess combined properties in terms of pH activity and thermostability profiles.

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