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Nicolaj Cruys-Bagger, Guilin Ren, Hirosuke Tatsumi, Martin J. Baumann, Nikolaj Spodsberg, Heidi Delcomyn Andersen, Lo Gorton, Kim Borch and Peter Westh An amperometric enzyme biosensor for real-time measurements of cellobiohydrolase activity on insoluble cellulose Biotechnology and Bioengineering 109

Article first published online: 25 JUL 2012 | DOI: 10.1002/bit.24593

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In this communication the authors show that the initial kinetics for cellobiohydrolase I, Cel7A from Trichoderma reesei, acting on different types of cellulose substrates, semicrystalline and amorphous, can be monitored directly and in real-time by an enzyme-modified electrode based on cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium.

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