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Xiuling Li, Pavel Přibyl, Kateřina Bišová, Shigeyuki Kawano, Vladislav Cepák, Vilém Zachleder, Mária Čížková, Irena Brányiková and Milada Vítová The microalga Parachlorella kessleri––A novel highly efficient lipid producer Biotechnology and Bioengineering 110

Version of Record online: 18 JUL 2012 | DOI: 10.1002/bit.24595

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Limitation with any macroelement or depletion of mineral medium stimulated lipid overproduction in the alga Parachlorella kessleri. The strategy for industrial application consists of the fast growth of culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW.

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