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Kok Hong Lim, Heng Huang, Arnd Pralle and Sheldon Park Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection Biotechnology and Bioengineering 110

Version of Record online: 8 AUG 2012 | DOI: 10.1002/bit.24605

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The obligate tetrameric structure of streptavidin prevents its use in some applications. Lim and coworkers engineered stable and high affinity monomeric streptavidin by introducing rational mutations based on its homolog, rhizavidin. The resulting mSA recognizes biotin as a structural monomer and can be recombinantly fused to other proteins to introduce a biotin binding tag.

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