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Catherine E.M. Hogwood, Andrew S. Tait, Nadejda Koloteva-Levine, Daniel G. Bracewell and C. Mark Smales The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process Biotechnology and Bioengineering 110

Article first published online: 6 AUG 2012 | DOI: 10.1002/bit.24607

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A proteomics analysis approach (2D-PAGE) has revealed that subsequent downstream processing choices following the harvest of the cell culture supernatant from Chinese hamster ovary cell lines expressing a model monoclonal antibody impacts upon the host cell protein (HCP) profile. We utilized an ultra scale down approach which could be used in early process development to aid the design/selection of clarification techniques to minimize HCP content and/or to screen potential cell lines to select those with overall reduced HCP profiles or of those HCPs which are problematic and challenging to remove.

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