Sneha Srikrishnan, Wilfred Chen and Nancy A. Da Silva Functional assembly and characterization of a modular xylanosome for hemicellulose hydrolysis in yeast Biotechnology and Bioengineering 110
Five modular xylanosomes displaying three fungal enzymes were assembled on the yeast cell surface for the synergistic saccharification of xylan. The synergy due to enzyme-enzyme and enzyme–substrate proximity, and the effects of binding domain choice and position on hydrolysis were determined. The xylanosome with a binding domain from Thermotoga maritima improved hydrolysis by 3.3-fold over free enzymes. Swapping the xylan binding module position within the scaffoldin increased hydrolysis 1.5-fold when the binding domain was adjacent to the endoxylanase.
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