E-mail

E-mail a Wiley Online Library Link

Sneha Srikrishnan, Wilfred Chen and Nancy A. Da Silva Functional assembly and characterization of a modular xylanosome for hemicellulose hydrolysis in yeast Biotechnology and Bioengineering 110

Article first published online: 8 AUG 2012 | DOI: 10.1002/bit.24609

Thumbnail image of graphical abstract

Five modular xylanosomes displaying three fungal enzymes were assembled on the yeast cell surface for the synergistic saccharification of xylan. The synergy due to enzyme-enzyme and enzyme–substrate proximity, and the effects of binding domain choice and position on hydrolysis were determined. The xylanosome with a binding domain from Thermotoga maritima improved hydrolysis by 3.3-fold over free enzymes. Swapping the xylan binding module position within the scaffoldin increased hydrolysis 1.5-fold when the binding domain was adjacent to the endoxylanase.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field

SEARCH