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Sneha Srikrishnan, Wilfred Chen and Nancy A. Da Silva Functional assembly and characterization of a modular xylanosome for hemicellulose hydrolysis in yeast Biotechnology and Bioengineering 110

Article first published online: 8 AUG 2012 | DOI: 10.1002/bit.24609

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Five modular xylanosomes displaying three fungal enzymes were assembled on the yeast cell surface for the synergistic saccharification of xylan. The synergy due to enzyme-enzyme and enzyme–substrate proximity, and the effects of binding domain choice and position on hydrolysis were determined. The xylanosome with a binding domain from Thermotoga maritima improved hydrolysis by 3.3-fold over free enzymes. Swapping the xylan binding module position within the scaffoldin increased hydrolysis 1.5-fold when the binding domain was adjacent to the endoxylanase.

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