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Aswan Al-Abboodi, Jing Fu, Pauline M. Doran and Peggy P.Y. Chan Three-dimensional nanocharacterization of porous hydrogel with ion and electron beams Biotechnology and Bioengineering 110

Version of Record online: 6 AUG 2012 | DOI: 10.1002/bit.24612

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The ability to image three-dimensional (3D) cell growth is crucial for understanding and studying various cellular activities in a 3D context, particularly for designing new tissue engineering scaffolds. The authors demonstrate using focused ion beam (FIB) milling, electron imaging, and associated microanalysis techniques such that novel 3D characterizations can be performed effectively on cells growing inside a 3D hydrogel scaffold. With FIB-tomography, the porous microstructures were revealed at nanometer resolution, with the cells grown inside.

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