Shiva Shanker Kaki and Patrick Adlercreutz Quantitative analysis of enzymatic fractionation of multiple substrate mixtures Biotechnology and Bioengineering 110
Enzymatic fractionation of multiple substrate mixtures can be quantitatively analyzed in a way similar to the established methodology used in enantiomer resolution. Based on competitive factors (α1, α2, etc., ratios of specificity constants), useful predictions can be made concerning the outcome of the reactions. Enrichment of erucic acid in mixtures with other fatty acids is used as an example.
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