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Jeremy S. Luterbacher, Jean-Yves Parlange and Larry P. Walker A pore-hindered diffusion and reaction model can help explain the importance of pore size distribution in enzymatic hydrolysis of biomass Biotechnology and Bioengineering 110

Version of Record online: 18 SEP 2012 | DOI: 10.1002/bit.24614

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A key, and often overlooked, role of biomass pretreatment is to increase the accessibility of the material to cell-wall-degrading-enzymes (CWDEs) such as cellulases. This accessibility is essential to expanding reactive surface areas for enzyme binding and subsequent hydrolysis and it is likely to affect the fraction of pore volume available for CWDEs to act synergistically. Thus, our accessibility model analyzes this contribution and is important for our understanding of how CWDEs interact with pretreated biomass.

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