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Kimio Sumaru, Kyoko Kikuchi, Toshiyuki Takagi, Manae Yamaguchi, Taku Satoh, Kana Morishita and Toshiyuki Kanamori On-demand killing of adherent cells on photo-acid-generating culture substrates Biotechnology and Bioengineering 110

Version of Record online: 22 AUG 2012 | DOI: 10.1002/bit.24617

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Novel method to kill adherent cells as cultured on a substrate by micro-projection of mild visible light was developed by means of culture substrates functionalized with photo-acid-generating polymer. Patterned killing and in situ screening of CHO-K1 cells were demonstrated by using a PC-controlled micro-pattern projection system. Further, photo-selective killing after photo-induced patterning of CHO-K1 cells was achieved on a substrate.

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