Jae-Hun Lee, Changhyeon Song, Do-Hyun Kim, Il-Hyang Park, Sun-Gu Lee, Yoon-Sik Lee and Byung-Gee Kim Glutamine (Q)-peptide screening for transglutaminase reaction using mRNA display Biotechnology and Bioengineering 110
The glutamine substrates of transglutaminase (TG) from Streptomyces mobaraensis can be rapidly and efficiently screened using mRNA display. Results demonstrate the screened substrates are short, specific and highly reactive for TG from Streptomyces mobaraensis. This method shows the significant potential to investigate the subsite specificities of unknown TGs and to screen their specific substrates.
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