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Kyung Ok Yu, Ju Jung, Ahmad Bazli Ramzi, Se Hoon Choe, Seung Wook Kim, Chulhwan Park and Sung Ok Han Development of a Saccharomyces cerevisiae strain for increasing the accumulation of triacylglycerol as a microbial oil feedstock for biodiesel production using glycerol as a substrate Biotechnology and Bioengineering 110

Version of Record online: 16 AUG 2012 | DOI: 10.1002/bit.24623

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The aim of this study is to present the accumulation of microbial oil for biodiesel feedstock from low cost feedstock. The authors demonstrated the overproduction of TAG from glycerol in engineered Saccharomyces cerevisiae via the glycerol-3-phosphate pathway by overexpressing the major TAG synthesis. The content of TAG in the engineered strain yielded 8.2% TAG, representing a 2.3-fold improvement compared to the wild-type strain. The results should allow a reduction of costs and a more sustainable production of biodiesel.

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