Son Q. Pham, Pengfei Gao and Zhi Li Engineering of recombinant E. coli cells co-expressing P450pyrTM monooxygenase and glucose dehydrogenase for highly regio- and stereoselective hydroxylation of alicycles with cofactor recycling Biotechnology and Bioengineering 110
A three-component P450pyrTM monooxygenase was successfully co-expressed with a GDH in E. coli as an efficient whole-cell biocatalyst for highly regio- and stereoselective hydroxylation of alicyclic substrates at non-activated carbon atoms. Enhanced productivity resulted via intracellular recycling of NAD(P)H, providing simple and useful syntheses of several alicyclic alcohols in high ee that are valuable pharmaceutical intermediates and difficult to prepare by other methods. E. coli (P450pyrTM-GDH) represents the most productive system known thus far for enhancing P450-catalyzed hydroxylations via cofactor recycling.
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