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Tobias Klein, Konstantin Schneider and Elmar Heinzle A system of miniaturized stirred bioreactors for parallel continuous cultivation of yeast with online measurement of dissolved oxygen and off-gas Biotechnology and Bioengineering 110

Version of Record online: 24 AUG 2012 | DOI: 10.1002/bit.24633

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Klein, Schneider, and Heinzle describe a system of 8 parallel bioreactors for continuous cultivation with only 10 mL working volume. Non-invasive optical measurement of dissolved oxygen ensures aerobic cultivation conditions and is a strong indicator of steady state. Stirring of the bioreactors improves oxygen transfer and prevents sedimentation of cells. Mass spectrometric off gas analysis permits determination of O2 uptake and CO2 production rates and further allows complete carbon balancing. Biological validation has been performed by cultivating fission yeast under various physiological conditions.

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