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Sigrid K. Hansen, Babak Jamali and Jürgen Hubbuch Selective high throughput protein quantification based on UV absorption spectra Biotechnology and Bioengineering 110

Version of Record online: 18 SEP 2012 | DOI: 10.1002/bit.24712

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A detailed study of the spectral differences and similarities of 26 proteins underlined the usefulness of spectral data for high throughput selective protein quantification. A measure of spectral similarity was defined and shown to correlate with the precision of selective protein quantification in protein mixtures based on their absorption spectra. Knowledge of this correlation can be used to estimate the precision of the method for a certain protein combination a priori.

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