Ricardo P. Baptista, David A. Fluri and Peter W. Zandstra High density continuous production of murine pluripotent cells in an acoustic perfused bioreactor at different oxygen concentrations Biotechnology and Bioengineering 110
Strategies for the production of pluripotent stem cells (PSCs) rely on serially dissociated cultures limiting robust scale-up of cell production, on-line control and optimization of culture conditions. Baptista, Fluri, and Zandstra demonstrate the scalable production of PSCs and early derivatives using acoustic filter technology to enable continuous oxygen-controlled perfusion culture. This work establishes a versatile biotechnological platform for the production of pluripotent cells and derivatives in an integrated, scalable, and intensified stirred suspension culture.
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